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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
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Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Journal: Scientific Reports

Article Title: Self-assembling thermostable chimeras as new platform for arsenic biosensing

doi: 10.1038/s41598-021-82648-9

Figure Lengend Snippet: Stability evaluation of immobilized ArsC-Vmh2 (red) and Vmh2-ArsC (blue). ( a , b ) Functional stability of ArsC-Vmh2 and Vmh2-ArsC stored at 4 °C up to 15 days. ( c , d ) Residual activity of ArsC-Vmh2 and Vmh2-ArsC after n assays on the same immobilized proteins. Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Statistical analysis was performed through the ordinary one-way ANOVA on GraphPad Prism 7.00; significant differences of TtArsC in comparison to chimeras are indicated as: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Techniques: Functional Assay, Activity Assay